Content
- Journal of Materials Chemistry B.
- Materials and methods
- Reagents and equipment
- Preparation of mesoporous radical BG nanoparticles
- Preparation of amino-modified rMBG (rMBG-NH2) and FITC-loaded rMBG (rMBG-FITC) nanoparticles
- Properties of rMBG nanoparticles
- Apatite mineral rMBGs
- Generate pOA-EGFP
- Zata standards
- Gene activation and maintenance of the rMBG gene• Cell culture and proliferation.
- rMBG particles enter the cell
- Cell contamination
- Results
- Advantages of rMBG
- Mining rMBG
- Function of rMNG cell entry units
- Gene tax
- Conservation Gene protection rMBGs
- rMBG cells
- Transfection of rMBGs gene
- Discussion
- Results
Preface
With the rapid development of bone grafting in recent years, osteoinductive bone repair materials have begun to be widely used in medicine. 1,2 Among these, bio ceramic materials such as hydroxyapatite (HA), calcium phosphate, bioactive glass (BG) and related materials are widely used in bone grafting. 3,4 Bioactive glasses, first developed by Hench and colleagues in 1969, has higher bone formation than other mineral substances such as-hydroxyapatite. 6.7 BG has become an important inorganic compound for bone regeneration and dental treatments. Importance is also given to the study of 3.8 BG.BG has good biocompatibility with biological minerals, thanks to chemical research carried out on the material. 7,9-11 ion release products released from BG can act on surface apatite, 12-14 upregulate osteogenic gene expression, 15-17 stimulate osteoblast proliferation and differentiation, 15,16 increase muscle growth secretion. (VEGF). 18-20 These natural and positive consequences of blood sugar are involved in bioactivity and promote rapid bone formation. Nowadays, BG is widely used in orthopedics and stomatology due to its bioactivity, angiogenic properties and good potential7,21. Considered the gold standard of BG, Bio glass 45S5 is a very large material with low specific surface area produced by conventional melting process. 4,8 However, the melting method has some limitations, such as high processing costs, the absence of microporous structures in the material, and specific surface area. 4.7 The sol–gel process, first reported in the early 1990s,
This journal is © Royal Society of Chemistry 2014.alternative ways to prepare BG. 22 The low-temperature sol–gel process offers many potential advantages over the melting process, including easier access to specific particle sizes, better quality control and bioactivity, and a larger specific surface area. 4.21About a decade ago, a new type of BG, called mesoporous bioactive glasses (MBGs), was synthesized by Yan and co-workers23 via the sol–gel method and supramolecular surfactant chemistry. MBGs are uniform and sufficiently porous, have controlled nanopore size and pore size, good cell compatibility, and mineral apatite. 24-26 Due to their excellent properties, studies of MBGs for biomolecular delivery and artificial bone production have become very popular and. 27,28 MBGs have been prepared as particles, domains, or other structures by combining material conductors with special techniques.
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